Abstract: To further optimize the alcoholization process of flue-cured tobacco leaves, an alcoholization experiment has been carried out for the C3F of three flue-cured tobacco varieties (CB-1, K326 and Yunyan 87). The result showed that the leaf chlorophyll content of all varieties decreased before reaching a stable level as the alcoholization went. The similar change was observed for carotenoid content but change more slowly. Changes in main chemical component of three varieties varied. The total nitrogen and nicotine content in CB-1 showed a sharp fall, while the total sugar content increased; however, the total nitrogen and nicotine content in K326 and Yunyan 87 increased slightly, while the total sugar content decreased. The reducing sugar content in all varieties increased as alcoholization processed, but CB-1 increased the most, followed by Yunyan 87 and then K326. The pH value of tobacco leaves gradually decreased in the process of alcoholization, and the alcoholization speed of CB-1 was obviously quicker than that of K326 and Yunyan 87.