Abstract: By using flue-cured tobacco variety K326 as a control, we investigated variety (Yunyan 105 and NC297) adaptability to various latitudes in Jiangxi province, analyzed and evaluated their major agronomic characters, economic characters and leaf quality. The results showed that Yunyan 105 grew fast in early stage, and moderate in middle and late stages of field growth. The appearance quality of Yunyan 105 was slightly better than that of K326. Yunyan 105 was easier to cure than K326 under the same cultivation and curing measures. The yield and production value of Yunyan 105 were significantly higher than those of K326, and its chemical composition was more coordinated than that of K326. NC297 grew moderately in the early stage and fast in the middle stage and final stage of field growth. The maturity of NC297 was better than that of K326. NC297 had strong comprehensive resistance, coordinated chemical composition, stable yield and quality features, and good adaptability. The yield and quality of NC297 were better than those of Yunyan 105 under Jiangxi Tobacco ecological conditions. Therefore, we recommend that NC297 as a potential variety to plant in a large area in Jiangxi province.