Abstract: Because tobacco leaf quality in Zhaotong could not meet the higher requirements of cigarette enterprises for raw tobacco quality, Field experiment were conducted to study the effect of adjusting fertilization on the tobacco growth and leaf quality from 2010 to 2011, including local conventional fertilization, adjusting the fertilizing method and adjusting the nutrients ratio. The results showed that, adjusting fertilizer method was the best in term of the agronomic attributes for the two years. Adjusting fertilizer method and adjusting nutrients ratio treatments improved disease resistance in 2010. Compared with conventional fertilizer application, adjusting fertilizer treatment increased yield and economic value in different levels, and the effect of adjusting nutrients ratio was better. There were little difference among leaf length, leaf width and leaf weight among treatments. Percentage of stem and leaf density of adjusting nutrients ratio was relatively better than others. Total sugar and reducing sugar for adjusting fertilizer treatment were higher than conventional fertilization. The two total scores for adjusting fertilizer method and adjusting nutrients ratio treatments increased by 4.1 and 3 points, respectively, and the quality improved by 2 grades. In conclusion, adjusting fertilizer method and adjusting nutrients ratio treatment can improve quality and increase yield, but the later treatment was more effectively.