Abstract: To search for substitutes of chemical fertilizers and chemical pesticides in the production of strong flavor type tobacco, biogas slurry was applied in Zhu Cheng. An Qiu and Gao Mi. The results showed that biogas slurry can promote the growth of tobacco seedlings, enhance the stem height, stem diameter and root weight. The biogas slurry also can improve the agronomic characters of tobacco. The yield, market value, average price and proportion of superior tobacco were increased by 4.02%, 8.59%, 4.38% and 8.15% respectively by biogas slurry application compared with the control. The appearance quality of tobacco however, was not changed. Biogas slurry showed good control effect on Myzus persicae as well as tobacco virus disease, black shank and brown spot, with the incidence rates of the 3 diseases were reduced by 59.96%, 55.56% and 19.05% respectively. In conclusion, biogas slurry can promote the growth of tobacco, control tobacco diseases and pests, and improve tobacco quality.