烟草脉带花叶病毒云南分离物全基因序列分析

Analysis of the complete genomic sequence of an isolate of Tobacco vein banding mosaic virus from Yunnan

摘要: 应用电子显微镜负染色法在烟草叶片斑驳、脉间褪绿病样中观察到马铃薯Y病毒属病毒粒子,通过马铃薯Y病毒属兼并引物扩增获得目的片段,序列分析表明病样中病原物为TVBMV,命名为YN-YX-TVBMV。根据GenBank已知序列,分别合成引物,对YN-YX-TVBMV进行克隆及测序,得到一条6047 bp的不完整的病毒基因组核苷酸序列,将该序列与国内报道的TVBMV全基因序列进行对比分析发现该分离物与报道的TVBMV其他分离物具有明显的地域分布差异,即云南获得的分离物均独立聚为一簇。

Abstract: The virus was first observed from Potyvirus with electron microscope. The virus was then identified as TVBMV by RT-PCR using primers M4 and Sprimers. The primers were designed using the genomic sequence of the 3' end of Potyvirus. The isolate was named YN-YX-TVBMV. A 6047 bp sequence was obtained from eight segments of YN-YX-TVBMV, This sequence was used to compare with TVBMV isolates from China. The results indicated that significant sequence variation existed among TVBMV isolates from diferent locations with the isolates from Yunnan Province always formed a separate group.