Abstract: Tobacco (Nicotiana tabacum L.) is densely covered with various types of glandular trichomes, which secrete a wide range of natural products. Tobacco glandular trichomes play important roles in stress responses and aroma components. Recently, significant progress has been obtained about the molecular mechanism of tobacco glandular trichome development. The paper reviews the current research progress on the tobacco genes involved in trichome development and trichome secretion synthesis. It provided useful information for the molecular regulation of trichome secretions and variety improvement.