Abstract: In order to survey the occurrence of tobacco viral diseases in Fengdu, Chongqing, 117 tobacco leaf samples with viral symptoms were collected at different growth stages (seeding, stem elongation, vigorous growing and mature) and detected with the Dot-ELISA technique using the specific antibodies against 7 viruses including TMV, CMV, PVY, TEV, PVX, TuMV and BBWV-2. Field investigation showed that virus-induced symptoms of tobacco include mosaic, leaf malformation, necrotic rings, vein necrosis, dwarf and etch. The incidence of tobacco virus diseases at nine survey spots is between 28%-84% and the disease index is between 6.67-60.89. The results revealed that no virus was detectable in samples at the seeding stage and PVX was undetectable in samples at the elongating stage. All of the 7 viruses were detected at the late growth stages. Both TMV and CMV had higher detection ratios than those of TuMV and PVY while PVX had the lowest detection ratio. These results showed that tobacco viral diseases commonly occur in Fengdu and TMV and CMV are the prevalent pathogenic viruses.