Abstract: a field experiment was conducted to sudy the effect of combined application of bean cake fertilizer and inorganic fertilizer on neutral aroma constituent contents, sensory quality, yield and output value of flue-cured tobacco by using cultivar NC55. The results showed that total neutral aroma constituent content and the neophytadiene content increased first, then decreased with the increase of bean cake fertilizer application, with the treatment with 50% bean cake fertilizer had the highest contents. The treatment with 100% bean cake fertilizer had the highest content of the neutral aroma constituents except for neophytadiene, while the treatment with 50% bean cake fertilizer had the lowest. The content of aroma constituents of carotenoids increased with the increasing ratio of bean cake fertilizer. The treatments with 50% and 100% bean cake fertilizer had the higher contents of aroma constituents of phenylalanine, Maillard reaction products and cembranenoids. The combined application of bean cake fertilizer improved the yield and output value of tobacco, with the treatment with 50% bean cake fertilizer had the highest yield, and the treatment with 75% bean cake fertilizer had the highest output value. Since the cost of fertilizers increased sharply with the increasing ratio of bean cake fertilizer, the income of the treatment with 50% bean cake fertilizer was the highest after cost removal. Therefore, in the production of flue-cured tobacco, the optimal combination ratio of bean cake fertilizer is 50% (N).