Abstract: In order to investigate the biological function of the tobacco pathogenesis-related protein PR10,NtPR10 gene was obtained from the tobacco variety G28,which is 483 bp in length,encoding 160 amino acids.The NtPR10 protein belongs to the pathogenesis related protein family containing the "Bet_v_I " domain and the " P-loop" domain which is related to nuclease activities.The bioinformatics analysis indicated that NtPR10 did not contain transmembrane region,no signal peptide,and exhibited intracellular localization features by TMHMM,SignalP and PrositeScan.The expression pattern of the gene in the resistant and susceptible cultivars was studied by RT-qPCR.The results showed that the NtPR10 gene was significantly up-regulated in the susceptible cultivar G28 after TMV treatment.While in resistant cultivar Sumsun NN,NtPR10 was up-regulated at 6 hours after TMV infection,and down-regulated between 12 hours and 8^th day,and then increased gradually to the pre-infection level after 16th day.From above analysis,NtPR10 responses to TMV infection,and the expression trend is opposite in resistant and susceptible cultivars,suggesting that the NtPR10 might have an important function in the process of TMV infection.Results from this study provide the foundation for further analysis of the biological function of NtPR10.