Abstract: The mixed use of fertilizers and pesticides is a new agricultural practice, but their effects on pesticide degradation is not investigated. In this study, a multi-residue analysis method based on QuEChERS and HPLC-MS/MS was developed and the field trials on different application patterns of pesticides and fertilizers were carried out to study the effects of foliar fertilizer on degradation rate of four pesticides, including chlorantraniliprole, flonicamid, flubendiamide and sulfoxflor. The results showed that the application of foliar fertilizer has a significant effect on promoting the degradation of chlorantraniliprole. Accompanied by there was a little effect on degradation of flonicamid, flubendiamide and sulfoxaflor, the best dilution concentration is 1000 multiple. The results provide the basic data for reducing of tobacco pesticide residues and improving the quality of tobacco leaves through the "combination of pesticide and fertilizer " model.