Abstract:
To reveal the fungal diversity of yellow sun-cured tobacco during curing, the composition and dynamics of fungal communities were analyzed by a combination of pure cultivation and molecular identification techniques. Cultivable fungi were isolated from the early, middle and late stages of curing. The representative strains were then phylogenetically analyzed based on ITS sequences. The results showed that a total of 126 fungal strains were isolated. The strains isolated from the middle leaves were relatively less compared with those from the bottom and upper leaves. In addition, the tobacco leaves seemed to contain more fungi at the early and late stages comparing to those at the middle stage of curing. The 126 fungal strains were distributed in 2 phyla, 5 classes, 10 orders, 14 families and 17 genera.
Ascomycota was the dominant phylum, with 93.65% of the isolates belonging to this phylum, and
Epicoccum, Arthrinium and
Fusarium were the dominant genera, accounting for 23.02%, 13.49% and 11.90% of the isolates, respectively. It was found that the fungal diversity of yellow sun-cured tobacco leaves during curing was relatively abundant, and the fungal composition varied in different leaf positions and dynamically changed as the curing process proceeded.
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