烟草过氧化氢酶基因<i>CAT2</i>克隆与表达特征分析

侯含; 王升平; 张超群; 周志成; 余祥文; 卢燕回; 贾海燕; 王杰; 李莹; 申莉莉; 王凤龙; 杨金广

doi:10.13496/j.issn.1007-5119.2019.01.001

烟草过氧化氢酶基因CAT2克隆与表达特征分析

Cloning of Catalase 2 (CAT2) Gene and Study on Its Expression Pattern in Nicotiana tabacum L.

摘要

摘要: 基于已有的植物Catalase 2基因（CAT2）序列设计烟草Nicotiana tabacum CAT2的特异性引物，通过RT-PCR扩增克隆获得Nicotiana tabacum var.NC89 CAT2全长mRNA序列，包含1479 bp完整的读码框（ORF），可编码492个氨基酸残基。遗传进化分析显示，烟草NC89 CAT2与N.benthamiana CAT2基因亲缘关系最近。利用Quantitative Real-time PCR技术分析了CAT2基因在NC89烟草中的组织特异性表达和其应对生物和非生物胁迫的表达差异。结果表明，CAT2基因在烟草NC89的根、茎、叶、花瓣、花萼、种子中均有表达，其中在花中相对表达量最高，其次为叶、茎、种子和根。生物和非生物胁迫处理烟株，其CAT2诱导表达分析显示，机械损伤、渗透压、低温和高温、干旱和感染PVY时CAT2表达量均上调。上述研究表明烟草CAT2基因可能参与了应对非生物和生物胁迫的过程。

Abstract: The full-length cDNA of Catalase 2 (CAT2) from Nicotiana tabacum var. NC89 was cloned usingspecific primers designed according to CAT2 mRNA sequences in other plants. Amino acid sequence alignment indicated that CAT2 contains a complete reading frame (ORF) of 1479 bp, which encodes 492 amino acid residues. Phylogenetic analysis showed that NC89 CAT2 shares high similarity with the CAT2 gene from N. benthamiana. Quantitative real-time PCR (qPCR) analysis revealed that CAT2 is highly expressed in petal and calyx, while less expressed in leaves, stems, seeds, and roots. The expression of NC89 CAT2 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought and PVY infection. It was suggested that CAT2 plays an important role in growth, development, and interplay of abiotic and biotic stresses in plant.

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