Abstract: Tobacco plant is quite vulnerable to abiotic stresses during its growth and development, which often results in the yield and quality decline of tobacco leaves. Thus it is urgent to enhance tobacco resistance to abiotic stress. The phytohormone abscisic acid (ABA) in tobacco can regulate various physiological processes and promote tobacco adaptation to abiotic stresses. ABA engineering might be one of the key strategies to obtain tobacco with tolerance to abiotic stress. This paper focuses on the research progress of ABA in tobacco, including general function, function in abiotic stress, metabolism and signaling pathway, etc., which might provide reference for the practical applications and research on tobacco stress-resistance breeding.