Abstract: In order to understand the genotoxicity of e-cigarettes and its evaluation methodology, a review was conducted according to the classification of study subjects. The test methods using bacteria as the study subjects include bacterial reversion mutation test and DNA damage analysis. Methods using isolated cells as study subjects include micronucleus test, DNA double-strand breaking test, RNA transcriptional sequencing test, targeted gene detection and analysis, and Bhas cell transformation test. Long-term inhalation toxicity test and reproductive development toxicity test were used in the model animals. The human body as the study subject are mainly clinical trials and epidemiological investigations. Due to the differences of study subjects and test methods, the credibility and comparability of e-cigarettes genotoxicity results is poor. Therefore, it is recommended that in the process of e-cigarettes genotoxicity evaluation, at least bacteria, isolated cell, animal models and clinical trials should be considered. Multiple genetic toxicity test endpoints can be obtained through a variety of test methods, such as reverse-mutation test, micronucleus test, long-term inhalation toxicity test, and clinical trial, which help to make a scientific, objective and comprehensive evaluation of e-cigarettes genotoxicity.