Abstract: In order to evaluate the ecological adaptability of different flue-cured tobacco varieties and to identify flue-cured tobacco varieties suitable for planting in the Bijie tobacco areas of Guizhou, using Yunyan 87 as a control, agronomic traits, natural disease rates, economic traits and sensory quality of Guiyan 202, Xiangyan 5, Yunyan 116, CC297 and CC27 were compared through filed experiments. The comprehensive traits of the varieties were evaluated in combination with a two-point regional test analysis and comprehensive index method. The results showed that Yunyan 116 performed better than the control at the two test sites, and showed strong adaptability to the environment. It was a broadly adaptable variety. CC27 performed better than the control at the Qianxi test site, but performed poorly at the Qixingguan test site. Guiyan 202 and Xiangyan 5 performed well at the Qianxi test site, and their performance at the Qixingguan test site was similar to the control. Average price, rate of first-class tobacco leaves, two-point regional test analysis in one year and comprehensive index evaluation of CC297 were worse than those of the control. The comprehensive performance of Yunyan 116 in terms of agronomic traits, natural disease rate in the field, economic traits and sensory quality was better than the control, and it was suitable for planting in Bijie tobacco areas.