Abstract: The present situation of two kinds of tobacco soil-borne diseases, black shank disease and root black rot disease, is increasing, which greatly aggravates the degree of tobacco diseases. In this study, two excellent biocontrol strains, Penicillium purpurogenum strain Q2 and Trichoderma asperellum strain MX, were used to combine with amino oligosaccharides, a plant inducer of disease resistance, respectively. Effects on tobacco growth promoting and control efficacy of tobacco black shank and black root rot have been investigated by two fungal biocontrol agents and their combination with amino oligosaccharides in the potting experiment. The results showed that P. purpurogenum strain Q2 and T. asperellum strain MX had strong antagonism against P. parasitica var. nicotianae and T. basicola, and the combination of them and amino oligosaccharides could better promote the growth of tobacco seedlings, and the control effect on the tobacco black shank and black root rot was more than 65%, which could significantly improve the activities of PAL, SOD and POD in the diseased tobacco.