Abstract: Hyperosmolal-gated calcium-permeable channel (OSCA) is a Ca²⁺ nonselective cation channel found in Arabidopsis thaliana.The OSCA family members contain a calcium-dependent channel DUF221 domain.In order to further understand the characteristics and functions of OSCA gene family members in tobacco,23 OSCA genes were identified from the genome of cultivated tobacco K326.Phylogenetic relationships,gene structures and protein domains,promoters,expression patterns in different tissues and organs,and response to drought and salt stresses of the NtOSCA family genes were analyzed.According to the evolutionary relationship, the NtOSCA gene family was divided into four subfamilies,and the protein and gene structure of the same subfamily members were similar.The results of promoter analysis showed that there were many different stress response elements in the promoters of NtOSCA family genes,among which the drought response elements and abscisic acid response elements were the most abundant.Further tissue expression analysis showed that the expression levels of NtOSCA family genes were different in different tissues.The expression pattern analysis of drought and salt stress responses showed that the expression levels of 20 NtOSCA genes were up-regulated under drought stress,and 15 NtOSCA genes were up-regulated under salt stress.These results indicated that OSCA gene family members played an important role in drought and salt stress resistance of tobacco.