Abstract: To further clarify the main virus species and infection type in Henan Province,407 samples were collected from nine tobacco-growing areas in Henan Province in 2020.The samples were suspicious of being infected by Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV),Potato virus Y (PVY) and Tobacco etch virus (TEV) respectively.Then four pairs of specific primers were used to detect viruses that infected tobacco samples by single RT-PCR.The results showed that the infection percentage of virus diseases in Henan tobacco-planting areas was 98.28%,in which CMV (89.19%) took the top spot followed by TMV (63.88%),PVY (28.99%) and TEV (24.32%).It also suggested that infection type included single infection and co-infection.What's more,single infection rate was 23.34%,in which CMV had the highest infection percentage with 16.71%.And co-infection percentage was 74.94% which was much higher than the single infection rate.Furthermore,there were three main co-infection types,including CMV+TMV with the highest infection percentage 36.86%,followed by CMV+TMV+PVY and CMV+TEV+PVY which both had an infection percentage of 7.62%.Therefore,CMV was the dominant virus in Henan Province in 2020.And the infection types were CMV+TMV, CMV+TMV+PVY and CMV+TEV+PVY,which indicated that co-infection types occurred seriously in tobacco field.