Abstract: To clarify the appearance regional characteristics of flue-cured tobacco from different aroma type zones and their relationship with sensory quality, 178 middle tobacco leaves of C3F grade in 2020 were collected from eight aroma type zones. The appearance regional characteristics of flue-cured tobacco were analyzed by the methods of quantitative assignment, variance analysis and cluster analysis. Besides, correlation and regression analysis was carried out on the relationship between appearance regional characteristics and sensory quality of tobacco leaves. The results showed that: (1) The background color and luster were the main identification bases for the aroma type V-VII zones; The wax texture was the main basis for identifying tobacco leaves from the aroma type VIII zone; (2) Tobacco leaves from the aroma type I-III zones were similar in background color, flexibility and leaf texture. The wax texture, luster and oily of tobacco leaves were similar in the aroma type V-VI zones. However, tobacco leaves from the aroma type IV and VIII zones were similar in thickness, leaf color difference between front and back, and oiliness. (3) The background color, wax texture and flexibility of C3F flue-cured tobacco leaves were significantly correlated with their sensory quality. In conclusion, the appearance regional characteristics of flue-cured tobacco from different aroma type zones have significant differences, which could provide the basis for the recognition and classified use of tobacco leaves.