低温胁迫下烟草开花调控基因表达分析

姜习振; 杨晨凯; 邹湘香; 李洋洋; 张庆富; 任晓敏; 晏伟杰; 姚未远; 贺新颖; 胡日生; 户正荣

doi:10.13496/j.issn.1007-5119.2023.03.002

低温胁迫下烟草开花调控基因表达分析

Expression Analysis of Flowering Regulatory Genes in Tobacco under Low Temperature Stress

摘要

摘要: 为了探究低温胁迫诱导烟草早花的分子机制，以低温敏感品种云烟87为试验材料，在12 ℃低温条件下处理烟苗14 d，取茎顶端组织进行烟草开花调控基因分析，并调查统计了烟株中心花开放时间和叶数。结果显示，与对照相比，低温胁迫导致烟株中心花开放时间平均提前7 d，叶数平均减少5片。基因表达分析结果表明，低温胁迫下开花调控基因NtMADS4，NtMADS11，NtAP1，NtSOC1，NtFUL，NtNFL1，NtFT1，NtFT3，NtFT4，NtFT5表达水平明显高于对照，在正常环境中恢复生长7～14 d，表达量下降至与对照相近的水平。以上结果表明，低温诱发烟草早花可能与这些基因上调表达有关。

Abstract: To investigate the molecular regulatory mechanism of low temperature induced early flowering, a cold-sensitive tobacco cultivar Yunyan 87 was treated with cold stress of 12 ℃ for 14 days. The shoot apical tissues of plants were collected for flowering-regulatory gene expression analysis. The time of central flower opening and the total number of leaves were investigated. The results showed that central flower opening date of the cold-treated tobacco plants was 7 days earlier than that of the control, and total leaf number of the treated plants was 5 leaves less than that of the control. Besides, the expression levels of 10 flowering-regulatory genes were increased by cold stress when compared to the control, including NtMADS4, NtMADS11, NtAP1, NtSOC1, NtFUL, NtNFL1, NtFT1, NtFT3, NtFT4 and NtFT5; and then decreased to similar levels of the control after recovery under normal conditions for 7-14 days. These results suggested that cold-induced tobacco early flowering may be related to the up-regulation of these flowering regulatory genes.

HTML全文

参考文献(25)

施引文献

资源附件(0)