Abstract: In order to select flue-cured tobacco varieties appropriate for planting in Central Area of Henan Province, and to select varieties that have high-quality, fitness for production, and fine resistance, field comparison test of six varieties were conducted. The growth periods, botanic characteristics, yields, leaf qualities, and tastes of six variety NC297, NC102, KRK26, KRK28, NC89 and zhongyan100 were studied at Pingdingshan, Henan province. The results showed that:(1) the growing stage, the plant height, the stem girth, the average leaf number, the node length of KRK26 and KRK28 were the biggest in all varieties，the agronomic characters of NC297 and NC102 were equal to NC89 and zhongyan 100 (CK1 and CK2). (2) KRK28 had the maximum yield, zhongyan100 had the maximum value and the highest proportions of top and middle class tobacco leaves, The yield and value of variety NC297 were higher than NC89 (CK1). (3) NC297, KRK26, NC89 had the best appearance quality; the inherent quality and smoking quality of NC297 and NC89 were better than other varieties. (4) The disease index against TMV and Black shank disease of the new varieties were all higher than NC89 (as the control), KRK26 had the best disease resistance in 6 varieties, NC89 had the worst disease resistance in 6 varieties. A comprehensive analysis on the agronomic traits, economic traits, inherent quality and disease resistance of different tobacco varieties, presented the best performing cultivars NC297, which should be recommended for further demonstration in central area of Henan province.