Abstract: Streamlining fertilizer processes, reducing labor intensity and increasing economic benefit of tobacco-growing were the purpose of this research. The field experiment was conducted to study application effects of one-time fertilizer in Huidong tobacco growing area. The results showed that tobacco with one-time fertilizer grew normally and maintained higher root activity and NR vitality. The dry weight of roots also increased. One-time fertilizer with KNO3 at the rate of 20 kg/ha reduced labor 27 person/ha and increased 3 685.9 yuan/ha. The chemical components contents were more coordinated, all in appropriate range of quality tobacco. It had great potential for popularization and application in Huidong tobacco growing area.