Abstract: The effects of nitrogen rates and leaves of tobacco remained on growth, yield, value and quality of flue-cured tobacco Zhongyan100 were studied by use of field experiments in Lichuan, Hubei province. The results showed that the effects of nitrogen rates and leaves of tobacco on growth condition in fields and yield were significant. The reducing sugar content, total sugar content, total plant alkaloid and other chemical composition in tobacco leaves between treatment were significantly different. The highest aroma quality, amount of aroma, aftertaste and total score were found treatment of 90 kg N/ha with 18-22 leaves remained.Therefore, this nitrogen rate and the number of leaves remained are recommended for tobacco production in this region.