Abstract: This study investigated the soil available N, N accumulation and accumulative rate of tobacco plants under different fertilizations. The results showed that the fertilizer nutrients from 0-40 cm soil loss completely in 40 days of fertilization in plots without tobacco. Net accumulation of soil available N occurred because mineralization of soil organic N exceed available N loss after 60 days. Soil available N content was obviously higher in chemical fertilizer treatment (D) than in inorganic/organic fertilizer treatment (G) in 63 days after transplanting under plastic muluching in all stage of tobacco plant growth. There was obviously negative correlation with N accumulation of tobacco plant and decline of soil available N. G treatment correlation (-0.935) was higher than D treatment (-0.661). Soil available N content was obviously higher in chemical fertilizer treatment (E) than in inorganic/organic fertilizer treatment (F) in 42 days after transplanting under removing film at bird nest stage. N accumulation and accumulative rate was higher in F treatment than in E treatment. Inorganic fertilizer treatment (I) was identical inorganic/organic fertilizer treatment (H) in soil available N in all stage of tobacco plant growth under advance fertilize and removing film, H treatment high than I treatment in tobacco plant N accumulation and accumulative rate. Inorganic fertilizer treatment had obviously higher than inorganic/organic fertilizer treatment in soil available N whatever fertilize mothed, and reverse in tobacco plant N accumulation. Therefore, Application of organic fertilization not only increased N accumulation in tobacco plant, but also increased plant N accumulative content and rate.