Abstract: The content and distribution of soil available micronutrients from tobacco-growing areas in Qingjiangyuan valley of southwest Hubei were studied and evaluated. The results showed that the content of available B and Zn were low, the content of available Cu, Fe and Mn were rich and the content of available Mo was the richest. However, the content of water soluble Cl was the lowest. The distribution of soil available micronutrient contents varied in Qingjiangyuan valley. The content of Fe, Mn and water soluble Cl were the highest, but Mo was the lowest in soils from Lichuan; The content of Cu and B were the highest and the content of Fe was the lowest in Xuan'en area; The content of Zn was the highest and the content B and water soluble Cl were the lowest in Enshi area; The content of Mo was the highest and Cu, Fe was the lowest in badong area; The content of Mn was the lowest in Xianfeng area. Therefore, B and Cl fertilizers should be applied more to tobacco-growing areas of Qingjiang valley. B Fertilizers should be increased in Enshi. Cl fertilizer should be increased in Enshi, Xuan'en, Badong, Xianfeng, Laifeng, especially Enshi.