Abstract: 272 of soil samples were collected from tobacco planting areas in Enshi. Soil pH, organic matter and macroelements were determined. The results indicated that about 90% of the soil pH was suitable for tobacco growth. In term of soil samples, 50%, 60% and 77.65% of soil organic matter, total N and available phosphorus content were suitable for tobacco growth. 35% and 60% of the soil s were found with relatively higher levels of the soil organic matter and available N. Nearly 23% of the soils available phosphorus content were extremely poorer. More than 60% of the soils available potassium content was deficient and quite diversity among different the regions. The contents of organic matter, available N, available phosphorus and available potassium increased with the elevation increased. Exchangeable magnesium contents was in general deficient and 72.6% of the soils were less than 0.4 cmol/kg. About 30.51% of the soils water-soluble B was deficient and 30.51% soils water-soluble B was deficiency. Soil available Zn contents of most soil samples was appropriate, but there have big difference in different sampling points.