Abstract: In order to make sustainable utilization of seeding medium in Huidong tobacco growing-area, crumbled and decayed corn stalk and slag were applied in the medium of tobacco float seeding system. The result showed that among various mixing proportion, the medium with 50% corn stalk + 20% slag + 20%fine perlite met the requirements of tobacco breeding medium, and the comprehensive characters of tobacco seedlings were the best. Therefore, it is feasible to use corn stalk and slag as a substitute in float seedling system, which could save 70% of ordinary seedling medium.