绒毛状烟草BAC文库的构建

BAC Library Construction of Villi Tobacco (Nicotiana tomentosiformis)

摘要: 绒毛状烟草是重要的野生种烟草之一。本研究以CopyControl^TM pCC1BAC^TM(Hind Ⅲ Cloning-Ready)Vector为载体,构建了绒毛状烟草的BAC文库。分析结果表明,BAC文库DNA插入片段为50~200 kb,平均110 kb,空载率<2%。随机挑选15个克隆进行稳定性继代试验,100代后插入片段仍然稳定存在。该绒毛状烟草BAC文库的建成,为进一步克隆重要功能基因提供了必备的物质平台。

Abstract: Villi tobacco (Nicotiana tomentosiformis) is an important species of wild tobacco. The BAC library was constructed with CopyControl^TM pCC1BAC^TM (HindⅢ Cloning-Ready) Vector. From the analysis of randomly selected BAC clones, the inserted DNA size of BAC library ranged from 50 to 200 kb; the average estimated was 110 kb; the empty clone was less than 2%; the insert was stable in a hundred generations.