Abstract: Plant growth-promoting rhizobacteria play an important role in plant growth. In order to investigate the effect of Brevibacillus brevis DZQ3 on tobacco growth and resistance of induction system, pot experiments were conducted in greenhouse and DZQ3 was inoculated into soaking root with filling root. Agronomic traits, biomass and chlorophyll content, leakage rate of electrolyte, systemic resistance related indices, including SOD, POD, PPO, PAL and CAT, and root development situation were measured. The results showed that the agronomic traits and biomass of tobaccos with application of DZQ3 were better than the control (CK), the difference were significant in 30 d. Systemic resistance related indices were better than the control (CK), the difference were significant in 60 d. These results indicate that PGPR strain DZQ3 can promote tobacco growth and induce system resistance, and also enhance disease resistant ability of tobaccos.