贵州黔南烟草马铃薯Y病毒(PVY)株系的血清学鉴定

Serological Identification of Strains of Potato Virus Y Infecting Tobacco Derived from Qiannan Guizhou Province

摘要: 2010年从贵州省黔南州烟草产区共采集201个表现明显马铃薯Y病毒病症状的烟草样本。采用Tas-ELISA检测PVY的3个株系PVY^N、PVY^O和PVY^C。结果显示, 201个样本中共检测出171个样本受到PVY侵染, 其中PVY^N 37个, 占21.6%;PVY^O 133个, 占77.8%;PVY^C 0个, PVY^N和PVY^O复合侵染1个, 占0.6%。PVY株系分布具有一定规律, 侵染黔南州烟草的PVY以PVY^O为主要株系, PVY^N侵染相对较少, 为次要株系, 所有样本均没有检测到PVY^C侵染, 复合侵染零星发生。

Abstract: Two hundred and one of tobacco samples with obvious PVY symptoms were collected from Qiannan in Guizhou Province, 2010. Three strains PVY^N, PVY^O and PVY^C of PVY were detected by Tas-ELISA. The results demonstrated that the 171 of 201 samples were infected with PVY, and strain PVY^N were 37, account for 21.6%; strain PVY^O were 133, account for 77.8%; strain PVY^C were 0, and complex infection of PVY^N and PVY^O was only one, account for 0.6%. The distribution regularity of PVY strains infecting tobacco in Qiannan was that the major strain of PVY was PVY^O, and PVY^N was a secondary strain, infecting relative less. No strain PVY^C was detected, and complex infection rarely occurred.