Abstract: In order to improve the quality of bulk curing of flue-cured tobacco, the effects of temperature rising rates (0.33 and 1℃/h) at leaf-drying stage on physio-biochemistry characters and quality were studied during bulk curing process by using electric-heated flue-curing barn. The result showed that, during yellowing stage, amylase activity was not much difference between the two treatments. However, after entering color fixing period, amylase activity was higher at slow heating rate than at fast heating rate. For both treatment, polyphenol oxidase activity first increased and then decreased. Polyphenol oxidase activity was lower with slow heating rate treatment than fast heating rate treatment at color fixing period. Chlorophyll and arytenoids contents declined more rapidly with slow heating rate treatment than with fast heating rate treatment. Rising temperature rate at leaf-drying stage had less impact on starch degradation. Slow heating rate was beneficial to increase the accumulation of total sugar and reducing sugar. The cured leaves at slow heating rate were better than fast heating rate in economic attributes, chemical components and smoking quality. Slow heating rate was better in general, and it was favorable to the quality improvement of cured leaves.