Abstract: Acid, neutral and alkaline aroma component contents of tobacco leaves in different ecological environment of Qiandongnan tobacco-growing region were analyzed, and the difference of all kinds of aroma components content in middle leaves of different varieties and leaves in different stalk position of same varieties were compared. The results showed that aroma component contents in tobacco leaves were higher in the eastern Qiandongnan than in the western Qiandongnan, indicating tobacco aroma contents was influenced by locations. The total aroma component content in K326 was the highest among all of the varieties, those of Yunyan 87 took the second place, and the content of Guiyan 201 was the lowest. The total aroma component content of middle leaves of Yunyan 85 and Yunyan 87 were higher than the upper leaves, and their flagrance were more prominent.