Abstract: In order to reveal the effect of nitrogen fertilization on basal aroma components and related character in different flue-cured tobacco, by use of variety 8306, Y892, K326, ZY100 as experimental material, dynamic content of chromoplast pigmen, leaf surface secretion and petroleum ether extract were determined in various nitrogen application rates. The results showed that four materials had the highest carotenoid content at medium nitrogen rate. Aroma material of 8306 and Y892 with higher aroma had higher carotenoid accumulation rate than K326 and ZY100. The leaf surface secretion in medium nitrogen rate was higher than that in low and high nitrogen treatments during leaf development. The leaf surface secretion of 8306 and Y892 was significantly higher than K326 and ZY100 in 40d of middle leaf ages. There were different nitrogen metabolism and accumulation in different genotypes of tobacco. The total contents of neutral aroma components increased with the increasing of nitrogen rate in the flue-cured leaves of 8306, K326 showed the inverse trend. Tobacco cultivation and management to improve the aroma should take different nitrogen application rates according to difference of genotypes to improve the aroma.