Abstract: In order to explore the best transplanting dates in high altitude area, an field experiment was carried out to investigate the effects of transplanting dates with plastic film on tobacco yield and leaf quality. The results showed that earlier transplanting increased seedling survival rate, and advanced every growth stages and leaf mature time about 5 days, and prolonged field growing time for 4 days, improved the agronomic properties, cured leaf appearance quality, the conformity of leaf chemical composition, the smoking quality, and economic indices of leaves. The early transplanting in high altitude region under plastic film provided better growth condition of plant and leaves, and resulted in normal mature and higher yield and better quality of leaves