Abstract:
RNA sequencing was used to investigate the differential expressed genes between cigar tobacco Beinhart 1000-1 and flue-cured tobacco Xiaohuangjin1025 under
Ralstonia solanacearum infection for clarifying the transcriptomic differences. The results showed that 568 preferentially expressed genes were identified in Beinhart 1000-1 at the infection stage I and enriched in carbohydrate metabolism and starch and sucrose metabolism pathways, while
1028 preferentially expressed genes were identified in Xiaohuangjin1025 and enriched in signal transduction and transcription factor pathways. Furthermore, 15 genes related to cell wall biosynthesis and remodeling and 3 members of
AP2/
ERF gene family were detected in the preferentially expressed genes of Beinhart 1000-1, while 3 and 24 genes were identified, respectively, in Xiaohuangjin1025. Besides, the biomass of
Ralstonia solanacearum in roots of Beinhart 1000-1 was much smaller than that in Xiaohuangjin1025. In all, this study showed that there were distinguished differences between Beinhart 1000-1 and Xiaohuangjin1025 in early transcriptional response to
Ralstonia solanacearum infection.
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