Sequence Analysis of the Ribosomal DNA ITS of <i>Phytophthora Parasitica</i> in Tobacco in Liangshan Region of Sichuan Province

ZHANG Haishan; XU Dafeng; LIU Dongyang; ZHOU Benguo; GAO Zhengliang; LU Jun; ZHANG Dongfang; YAN Dankan; WANG Fang; WANG Yong

doi:10.13496/j.issn.1007-5119.2015.01.018

CHINESE TOBACCO SCIENCE > 2015 > 36(1): 96-101. > DOI: 10.13496/j.issn.1007-5119.2015.01.018

ZHANG Haishan, XU Dafeng, LIU Dongyang, ZHOU Benguo, GAO Zhengliang, LU Jun, ZHANG Dongfang, YAN Dankan, WANG Fang, WANG Yong. Sequence Analysis of the Ribosomal DNA ITS of Phytophthora Parasitica in Tobacco in Liangshan Region of Sichuan Province[J]. CHINESE TOBACCO SCIENCE, 2015, 36(1): 96-101. DOI: 10.13496/j.issn.1007-5119.2015.01.018

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Sequence Analysis of the Ribosomal DNA ITS of Phytophthora Parasitica in Tobacco in Liangshan Region of Sichuan Province

1. Institute of Plant Protection and Agricultural Product Quality and Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China;
2. Tobacco Research Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China;
3. Liangshan Tobacco Company of Sichuan Province, Xichang, Sichuan 615000, China

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Received Date: May 05, 2014
Revised Date: December 02, 2014
Available Online: November 30, 2023

Graphical Abstract

Abstract

Abstract

The internal transcribed spacer regions (ITS) of the ribosomal RNA gene from 20 isolates of tobacco black shank were amplified with the polymerase chain reaction through the universal primers and sequence.The result showed that the total length of ITS of those 20 isolates tested were all 803bp, and the nucleotide sequence identity among those sequences was above 99.3%, and 20 strains had 99.6%-100% homologies with Phytophthora parasitica(GU111675.1) published in GenBank.But the ITS of those 20 isolates tested had some mutation sites which mainly concentrated in the ITS2 area and 468 mutation of isolates, which mainly came from Dechang and Xichang had certain regional.With the bio-software of MEGA6, Sequences from the above isolates were compared with 15 sequences published in GenBank database and phylogenitic trees were constructed based on ITS sequence data.The results showed that the isolates tested, GU111675.1, KF010303.1, AJ854295.1, KC768775.1 and L41383 were clustered into the same clade and all were Phytophthora parasitica (or Phytophthora nicotianae).They were clustered in a distant clade for different Phytophthora species and phylogenetically distinct each other.The results indicated that twenty isolates which isolated from different regions of Liangshan prefecture were Phytophthora parasitica.
- tobacco black shank,
- internal transcribed spacer regions,
- Phytophthora parasitica,
- tobacco

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References (15)

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