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    LI Yang, TANG Xuebing, LI Xiaofeng, LI Ming, CHU Zhaohui, DING Xinhua. The Influence of NtC3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco[J]. CHINESE TOBACCO SCIENCE, 2016, 37(1): 8-13. DOI: 10.13496/j.issn.1007-5119.2016.01.002
    Citation: LI Yang, TANG Xuebing, LI Xiaofeng, LI Ming, CHU Zhaohui, DING Xinhua. The Influence of NtC3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco[J]. CHINESE TOBACCO SCIENCE, 2016, 37(1): 8-13. DOI: 10.13496/j.issn.1007-5119.2016.01.002

    The Influence of NtC3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco

    • To test whether NtC3H participates in the biosynthesis of flavonoids, and chlorogenic acid (CGA) in tobacco and increases the contents of these secondary metabolites, we constructed the over-expression vector pCXSN::NtC3H and introduced it into Nicotiana tabacum var. samsun via Agrobacterium-mediated transformation. The concentrations of the different secondary metabolites including CGA, rutin, and kaempferol rutinoside in leaves of wild-type and transgenic lines were determined by HPLC. Compared to wild-type samsun tobacco, in transgenic tobacco the concentrations of CGA were increased 3.6-fold, rutin and kaempferol rutinoside were increased 6.1- and 24.6-fold respectively. These results suggested that NtC3H positively regulated the synthesis of flavonoids and CGA in tobacco.
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