Purification of a Protein Elicitor from Colletotrichum destructivum and Its Biological Activity in Inducing Tobacco Resistance and Facilitating Growth

To functionally investigate the mechanisms of disease resistance and tobacco growth, we have isolated a protein elicitor from mycelium of Colletotrichum destructivum by heating, centrifugation, precipitation, dialysis and gel chromatography column. We then measured the prevention and protection effects of this protein using spray and friction inoculation methods. The results showed that the protein elicitor appeared in Pro-P2-TX and the molecular weight was approximately 38 kD on silver-stained SDS-PAGE. The inhibitory effect to tobacco mosaic virus(TMV)was 73.79%. The protein elicitor could remarkably increase the individual plant height by 72.43%, leaf width by 61.06% and stem circumference by 29.05% respectively. Taken together, the protein elicitor has the role of inducing tobacco resistance and facilitating growth.