Screening and Application of Pseudomonas syringae pv. Tabaci-specific Primers

Tobacco wildfire, caused by Pseudomonas syringae pv. tabaci, is an important leaf tobacco bacterial disease. In order to quickly and accurately detect this disease, we downloaded the genome sequences from NCBI, including 25 Pseudomonas genus and 127 pathovars of P. syringae, and made a blast with Mauve 2.3.1 to find the specific fragments for P. syringae pv. tabaci. Based on this result, 13 primer pairs were designed with Primer Premier 6.0 and study their specificity for P. syringae pv. tabaci. One primer pair,40429, was picked out and verified with wildfire strains of different provinces. These results showed that primer 40429 could separate P. syringae pv. tabaci from other Pseudomonas genus and other pathovars of P. syringae by a single PCR product of 203 bp. It is indicated that the primer has a good applicability to the detection of P. syringae pv. tabaci and can be used in molecular identification.