Screening and Assisted Breeding Applications of SSR Molecular Markers of Sugar Esters in Tobacco

In order to clarify the usability of SSR molecular markers closely linked to sugar esters in the actual breeding of tobacco, and to accelerate the genetic improvement of flue-cured tobacco sugar esters, in this study, using polyacrylamide gel electrophoresis and GC-MS detection techniques, 5 tobacco germplasms with different genetic backgrounds and a genetic population with 192 recombinant inbred lines were used as experimental materials for screening and testing of 5 SSR molecular markers. The results showed that, among the 5 molecular markers, the polymorphisms of 2 molecular markers PT20315 and PT30354 were stable in different genetic backgrounds, and the correspondence between their polymorphic bands and the phenotypic difference of sugar esters were better. In the recombinant inbred line population, the consistency rate between the genotypes identified by the 2 molecular markers and phenotypes of sugar esters reached 93.75% and 90.58% respectively. The 2 molecular markers could be used in the molecular-assisted breeding of high-sugar ester tobacco. Currently, using of the 2 molecular markers in assisted selection has directionally improved new lineage materials such as high sugar ester K326 etc.