Screening, Identification and Combination of High Efficiency Degrading Bacteria for Tobacco Stem

In order to improve the degradation efficiency of lignin and cellulose in tobacco straw and shorten the decomposing time of tobacco straw, the strains with the ability to degrade lignin and cellulose were screened out by plate screening and enzyme activity determination experiments. The effects of different strain combinations on tobacco straw degradation were discussed. The results showed that the obtained Lysinibacillus fusiformis (N019a), Trametes hirsuta (MA), and Rhizopus oryzae (imp) were effective in degrading lignin, hemicellulose, and cellulose in tobacco straw. The composite flora I composed of N019a, MA and imp was used for solid fermentation of tobacco stalk powder for 25 d. The degradation rates of hemicellulose, cellulose and lignin were 67.23±0.73%, 61.17±0.45% and 60.12±0.48%, respectively. B. tequilensis (B4), B. subtilis (B26) and A. niger (M90) composed a complex flora II with degradation rates of hemicellulose, cellulose and lignin being 72.15±0.35%, 65.68±0.55%, and 38.15±0.76%. The degradation rates of hemicellulose, cellulose and lignin by compound flora III composed of 6 strains was were 83.32±0.45%, 75.21±0.71% and 66.13±0.53%. Different degrading bacteria degrade different components in wood fiber with certain specificity, and the combination of multifunctional strains can significantly improve the biomass degradation effect of tobacco straw. The research results provide a basis for the development of a highly effective compound bacterial agent for tobacco straw degradation.