Effects of Exobasidium Japonicum Liquid Culture on Growth and Key Enzyme Activities in Carbon and Nitrogen Metabolism of Tobacco Seedlings

To study the effects of Exobasidium japonicum liquid culture on growth and key enzymes of carbon and nitrogen metabolism of flue-cured tobacco seedlings, with K326 as the test material, different concentrations (original solution, 1/10 original solution, 1/50 original solution and 1/100 original solution) of the liquid culture were used to spray the flue-cured tobacco seedlings, the effects on growth, chlorophyll content, root activity and key enzyme activities of carbon and nitrogen metabolism of flue-cured tobacco seedlings were analyzed. The results showed that on the third day after treatment, the root activity of seedlings treated with 1/50 original solution was the highest, which was 276 μg/(g·h) higher than that of CK. Chlorophyll a content, chlorophyll b content and carotenoid content were increased by 0.324, 0.104 and 0.060 mg/g respectively. On the 7th day after treatment, the activities of NR, GS, SSS, SPS and INV of seedlings treated with 1/100 original solution were the highest, which were significantly increased by 0.36, 0.44, 5.16, 58.02 and 362.60 U/g compared with the control. On the 11th day after treatment, leaf length, leaf width, leaf area and plant height of seedlings treated by 1/100 solution were significantly higher than those treated by CK, increased by 3.11 cm, 1.03 cm, 25.39 cm² and 5.13 cm, respectively. Different concentrations of E. japonicum liquid culture had different effects on tobacco seedlings. In summary, 1/100 original solution concentration promoted the growth of flue-cured tobacco seedlings with the most significant effect, followed by 1/50 original solution.