Cloning and Sequence Analysis of NtRAX Gene in Tobacco

Based on Arabidopsis RAX gene sequence in GenBank, we conducted a Blastp search in tobacco EST database, and acquired a high homology with the protein sequence, then selected the highest protein sequence homology to the corresponding nucleic acid sequences to design primers, obtained the middle fragment, then used it to design primers, obtained the 5 'and 3' ends of the cDNA sequence by RACE strategy, a gene named NtRAX (NCBI accession number JQ228591) was obtained by splicing three cDNA sequences and sequencing full-length cloning. The full-length cDNA of NtRAX is 1112bp long with 96bp 5′-UTR, 62bp3′-UTR and 954bp coding sequence. The deduced amino acid is 641 AA long. Predicted molecular weight is 35.42KDa. NtRAX was predicted a tobacco axillary meristem formation regulatory genes with transcriptional activation activity,belonged to the MYB gene family.