Isolation, Purification and Antagonism of Extracellular Protein from Bacillus subtilis Strain SH7
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Graphical Abstract
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Abstract
Bacillus subtilis strain SH7 showed antagonism against Ralstonia solanacearum.One of the protein was purified by using ammonium sulfate precipitation followed by DEAE Sepharose Fast Flow chromatography and Sephadex G-75. The molecular mass of the protein was 33.6 kD by PAGE analysis. The active protein from Bacillus subtilis strain SH7 have diastatic activity.
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