Abstract: In order to provide a theoretical basis for accurate judgment of harvest maturity degrees of tobacco leaves, this study investigated changes of color parameters and pigment contents of flue-cured tobacco leaves during maturing process and their correlational relationship. The color parameters of flue-cured tobacco leaves of four different maturities were determined by color difference meter, and pigment contents was determined by spectrophotometer. This study used analysis of variance and simple correlation analysis. The results showed that lightness value (L^*), redness value (a^*) and yellowness value (b^*) of flue-cured tobacco leaves of various parts showed ascending tendencies during maturing process. There were different significances of differences of color parameters among four treatments with different maturities of tobacco leaves of various parts. During maturing process, variation tendencies of L^*, a^* and b^* of flue-cured tobacco leaves of various parts are different, so the variation trends of color parameters were different. And a^*of middle and upper leaves showed a larger. The contents of chlorophyll-a, chlorophyll-b, total chlorophyll and carotenoid all showed decreasing tendencies during maturing process, and the ratios of carotenoid and chlorophyll showed a ascending tendency. The lower and upper leaves kept almost simple decreasing amplitudes, but ratios of carotenoid and chlorophyll of middle leaves whose color changing area were between 70%-80% and 80%-90% showed a larger ascending tendency. The color parameters had highly or not highly significant correlation with contents of various pigments and the ratios of carotenoid and chlorophyll. The changes of contents of various pigments can be indicated by the color parameters, so the colorimeters can be supposed to be an auxiliary mean to judge the maturity of tobacco.