Abstract: Field investigation and laboratory analysis were conducted to study the distributive characteristics and relationships of micronutrients between soil and flue-cured tobacco of Shandong Province, thus, to provide a theoretical basis for the flue-cured tobacco production. The results showed that the contents of available Fe and Mn in the plough horizon were plentiful, while those of Cu and Zn were moderate and insufficient, respectively; in most tobacco-growing areas of Shandong, the Fe and Mn contents were in the range of high quality tobacco production in China, and the Cu and Zn contents were moderate. Multiple linear regression analysis and partial correlation analysis showed that the micronutrient contents in tobacco leaves were positively correlated with the available micronutrient contents in soil, there existed synergistic and antagonistic effects among the micronutrients. Therefore, Cu and Zn fertilizers should be applied more in Shandong.