Abstract: In order to protect tobacco soil ecological environment, improve the quality of tobacco, ensure the safety of tobacco, this article researched on the effects of different bio-organic fertilizers to the growth and development, yield and quality of flue-cured tobacco. The results showed that the oil cake, Qingjiangyuan and Jinfengye bio-organic fertilizers promoted the development of middle and upper leaves; enhanced the disease resistance; the yield enhanced 361.35, 218.85 and 17.55 kg/ha, and output value increased by 7744.95, 4838.40, 2257.80 yuan/ha; improved flue-cured tobacco chemical composition. Qingjiangyuan and Jinfengye bio-organic fertilizers are suitable for use in the production of tobacco in Enshi tobacco-growing areas.