Abstract: A field experiment with three harvest times and five nitrogen rates was conducted to study the effects of harvest times on physical, economic and chemical characters and the content of the pigment in the upper leaves of flue-cured tobacco. The results showed that the contents of total sugar and reducing sugar decreased with the postponement of harvest time. Nicotine content increased and then decreased when the nitrogen rate was <30 kg/ha, and it increased when the nitrogen rate was 30-60 kg/ha. Delayed harvest was not suitable if nitrogen rate was <30 kg/ha. Five days delayed harvest was the best when the nitrogen rates was 30, 45, and 60 kg/ha. The average price, proportion of high grage tobacco ratio and the sensory quality were the best in the five day delayed harvest with nitrogen rate of 45 kg/ha.