马里兰烟病毒病症状类型、毒源种类及病毒侵染构型研究

Classification of Virus Disease Symptoms, Kinds of Virus Species and Types of Infection of Virus on Maryland Tobacco

摘要: 湖北五峰县是中国唯一的马里兰烟种植区。2011年在湖北马里兰烟产区进行病毒病发生情况大田调查，发现主要有花叶、畸形、脉坏死、蚀刻等4种症状类型，并采用指示植物法对主要症状类型所含病毒的种类进行了鉴定；对采集的57份病毒病样本采用DAS-ELISA进行病毒种类的鉴定。结果表明，发生在马里兰烟上的烟草病毒种类包括TMV、CMV、PVX、PVY和TEV，TMV检出率最高，PVY次之；以2种或2种以上病毒复合侵染方式为主，其中TMV+PVY、TMV+CMV+PVY共存方式较为常见。

Abstract: Wufeng county in Hubei province is the only Maryland tobacco planting area in China. Maryland tobacco virus diseases were investigated there in 2011. The symptom types of virus diseases could be divided into 4 main kinds, including mosaic, distortion, vein necrosis, etching, which were identified through indication plants for virus species. DAS-ELISA was applied to identify the virus species to the collected 57 samples. It demonstrated that the virus species which infected Maryland tobacco included TMV, CMV, PVX, PVY and TEV. The detection rate of TMV was maximal among all the tested samples, and PVY took the second place. Meanwhile it commonly occurred that two and more kinds of virus co-existed, and TMV+PVY, TMV+CMV+PVY were the main patterns.