Abstract: A field experiment was carried out to study nutrient utilization and management in a rotational cropping system by using leaching chambers through analyzing nutrient utilization, product value and disease occurrence of tobacco. The results showed that there were no significant differences in yield, output and disease occurrence of tobacco between tobacco-maize rotation system and continuous tobacco cropping system with conventional fertilization. During a rotational cropping cycle, the inputs of N, P, and K were excessive. After reducing the fertilizer rate of corn in growing season, soil nutrients in rotation system maintained dynamic balance, and the yield of maize was stable, and the yield of tobacco maintained high level, the disease occurrence of tobacco was low.